Song-learning neurons identified in songbirds

October 21, 2019 , Hokkaido University
The zebra finch used in the study. Credit: Hokkaido University

A group of brain cells, the corticobasal ganglia projecting neurons, are important for vocal learning in young birds, but not in adult birds, according to a study published in the journal Proceedings of the National Academy of Sciences (PNAS).

Birdsong, like , is a series of precisely timed movements learned by copying the vocalizations of other individuals, such as parents. "The song system in birds shares a number of similarities with mammalian motor circuits," says Kazuhiro Wada, the Hokkaido University neuroscientist who led the study.

In birds, the song learning process depends on specific brain regions called the motor cortical area and the basal ganglia—these regions are also found in the mammalian motor system and are important for learning and maintaining sequential movements. However, it is unknown what role the neural connections between these two regions play.

To investigate this, the researchers studied zebra finches, a type of songbird, and explored what happens when the neurons that connect the motor cortical area and the are disrupted. They injected a virus which expresses cytotoxic protein into the connecting neurons. The toxins only killed these connecting neurons, which are called corticobasal ganglia projection neurons.

A birdsong sung by a zebra finch with disrupted projection neurons. Credit: Hokkaido University

They found that juvenile with disrupted projection neurons could not copy song patterns and developed unusual songs with inconsistent sequences. When they repeated the same experiments in adult birds, they found that birds with disrupted projection neurons did not alter their pre-learned song structure and showed normal vocal fluctuations.

The neurons connecting the motor cortical area and the basal ganglia in the brain were disrupted using viral toxins (left: normal, right: disrupted). This interrupted normal song learning in juveniles but not adults. Credit: Miguel Sánchez-Valpuesta et al., PNAS, Oct. 21, 2019.

The results imply that the corticobasal ganglia projection neurons are important for learning the acoustic and temporal aspects of a song's structure, but not for maintaining an already learned vocal pattern.

"Studying the corticobasal ganglia circuit may enhance our understanding of vocal development and communication disorders in humans," says Kazuhiro Wada.

More information: Miguel Sánchez-Valpuesta el al., "Cortico-basal ganglia projecting neurons are required for juvenile vocal learning but not for adult vocal plasticity in songbirds," PNAS (2019). www.pnas.org/cgi/doi/10.1073/pnas.1913575116

Journal information: Proceedings of the National Academy of Sciences

Provided by Hokkaido University